Overview

  • Product nameAnti-TSH Receptor antibody [4C1]
    See all TSH Receptor primary antibodies
  • Description
    Mouse monoclonal [4C1] to TSH Receptor
  • SpecificityAb27974 recognises TSH receptor.
  • Tested applicationsSuitable for: IP, Flow Cyt, IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: ELISA
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: External domain of human TSH receptor (tagged)

  • EpitopeAb27974 reacts with an epitope located in the extracellular region of TSH receptor.
  • Positive control
    • Human Thyroid gland.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesAb27974 was purified by affinity chromatography, from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone number4C1
  • MyelomaNS1/1-Ag4-1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/1000. Use 10µl of the suggested working dilution for 106 cells.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 87 kDa.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not required.
  • Application notesIs unsuitable for ELISA.
  • Target

    • FunctionReceptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
    • Tissue specificityExpressed in the thyroid.
    • Involvement in diseaseDefects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).
      Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation.
      Hypothyroidism, congenital, non-goitrous, 1
      Familial gestational hyperthyroidism
      Hyperthyroidism, non-autoimmune
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
      Contains 7 LRR (leucine-rich) repeats.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHNG1 antibody
      • hTSHR I antibody
      • hTSHRI antibody
      • LGR 3 antibody
      • LGR3 antibody
      • MGC75129 antibody
      • Seven transmembrane helix receptor antibody
      • Thyroid adenoma hyperfunctioning antibody
      • Thyroid carcinoma with thyrotoxicosis antibody
      • Thyroid stimulating hormone receptor antibody
      • Thyroid stimulating hormone receptor, isoform 2 antibody
      • Thyroid-stimulating hormone receptor antibody
      • Thyrotropin receptor antibody
      • Thyrotropin receptor I antibody
      • Thyrotropin receptor I, hTSHR I antibody
      • TSH R antibody
      • TSH-R antibody
      • TSHR antibody
      • TSHR_HUMAN antibody
      see all

    Anti-TSH Receptor antibody [4C1] images

    • ab27974 staining TSH Receptor in Human thyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    References for Anti-TSH Receptor antibody [4C1] (ab27974)

    This product has been referenced in:
    • Guest PC  et al. Proteomic analysis of the maternal protein restriction rat model for schizophrenia: identification of translational changes in hormonal signaling pathways and glutamate neurotransmission. Proteomics 12:3580-9 (2012). Read more (PubMed: 23071080) »
    • Romagnani P  et al. Expression of IP-10/CXCL10 and MIG/CXCL9 in the thyroid and increased levels of IP-10/CXCL10 in the serum of patients with recent-onset Graves' disease. Am J Pathol 161:195-206 (2002). Read more (PubMed: 12107104) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"