Overview

  • Product nameAnti-TSPAN2 antibody
    See all TSPAN2 primary antibodies
  • Description
    Mouse monoclonal to TSPAN2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Rat, Cow, Dog, Human, Pig, Chimpanzee
  • Immunogen

    Recombinant fragment: GKGVAIRHVQ TMYEEAYNDY LKDRGKGNGT LITFHSTFQC CGKESSEQVQ PTCPKELLGH KNCIDEIETI ISVKLQ, corresponding to amino acids 112-188 of Human TSPAN2 (NP_005716) with tag.

  • Positive control
    • Recombinant tagged human TSPAN2 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    sELISA: Use at a concentration of 10 µg/ml. Detection limit for recombinant tagged TSPAN2 is approximately 0.3ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTSPAN2 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. TSPAN2 may play a role in signalling in oligodendrocytes in the early stages of their terminal differentiation into myelin-forming glia and may also function in stabilizing the mature sheath.
    • Cellular localizationMembrane; Multi-pass membrane protein.
    • Database links
    • Alternative names
      • 6330415F13Rik antibody
      • AW048009 antibody
      • B230119D02Rik antibody
      • FLJ12082 antibody
      • OTTHUMP00000013651 antibody
      • RP4-666F24.2 antibody
      • Tetraspan 2 antibody
      • Tetraspan TM4SF antibody
      • Tetraspanin 2 antibody
      • Tetraspanin2 antibody
      • TSN2 antibody
      • Tspan 2 antibody
      see all

    Anti-TSPAN2 antibody images

    • Anti-TSPAN2 antibody (ab77105) at 1 µg/ml + Recombinant tagged human TSPAN2 fragment at 0.2 µg

      Secondary
      Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 24 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    References for Anti-TSPAN2 antibody (ab77105)

    ab77105 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77105.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"