Overview

  • Product nameAnti-TSPAN3 antibody
    See all TSPAN3 primary antibodies
  • Description
    Rabbit polyclonal to TSPAN3
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region of Human TSPAN3 (NP_944492)

  • Positive control
    • Hela cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceTSPAN3 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Two alternative transcripts encoding different isoforms have been described.
  • Cellular localizationMembrane
  • Database links
  • Alternative names
    • Tetraspan 3 antibody
    • Tetraspan TM4SF antibody
    • tetraspanin 3 antibody
    • tetraspanin TM4 A antibody
    • TM4 A antibody
    • TM4A antibody
    • TM4SF8 antibody
    • transmembrane 4 superfamily member 8 antibody
    • TSPAN 3 antibody
    • TSPAN3 antibody
    see all

Anti-TSPAN3 antibody images

  • Anti-TSPAN3 antibody (ab81018) at 1 µg/ml + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab81018 stained Hek293 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81018, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-TSPAN3 antibody (ab81018) at 1 µg/ml

    Lane 1 : non-reduced GST–tetraspanin EC2 expressed in E. coli
    Lane 2 : reduced GST–tetraspanin EC2 expressed in E. coli

    Lysates/proteins at 1 µg per lane.


    Predicted band size : 28 kDa

References for Anti-TSPAN3 antibody (ab81018)

ab81018 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"