Overview

  • Product nameAnti-TSPYL1 antibody
    See all TSPYL1 primary antibodies
  • Description
    Rabbit polyclonal to TSPYL1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 - 213 of Human TSPYL1

  • Positive control
    • WB: MOLT4 and Raji cell lysates IF: HeLa cells IHC-P: NCIN87 Xenograft

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Tissue specificityExpressed in testis, ovary, liver, spleen, brain, kidney, prostate, lung, liver, and heart.
  • Involvement in diseaseDefects in TSPYL1 are the cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT) [MIM:608800]. SIDDT is an autosomal recessive disorder. Affected infants appear normal at birth, develop signs of visceroautonomic dysfunction early in life, and die before 12 months of age of abrupt cardiorespiratory arrest. Features included bradycardia, hypothermia, severe gastroesophageal reflux, laryngospasm, bronchospasm, and abnormal cardiorespiratory patterns during sleep. Genotypic males with SIDDT had fetal testicular dysgenesis and ambiguous genitalia, with findings such as intraabdominal testes, dysplastic testes, deficient fetal testosterone production, fusion and rugation of the gonadal sac, and partial development of the penile shaft. Female sexual development was normal. Affected infants had an unusual staccato cry, similar to the cry of a goat.
  • Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SIDDT antibody
    • Testis specific like protein Y encoded antibody
    • Testis specific Y encoded like protein 1 antibody
    • Testis-specific Y-encoded-like protein 1 antibody
    • TSPY like antibody
    • TSPY like 1 antibody
    • TSPY like protein 1 antibody
    • TSPY-like protein 1 antibody
    • TSPYL antibody
    • TSPYL1 antibody
    • TSYL1_HUMAN antibody
    see all

Anti-TSPYL1 antibody images

  • All lanes : Anti-TSPYL1 antibody (ab95943) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 49 kDa
  • ab95943, at a 1/200 dilution, staining TSPYL1 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
  • ab95943, at a 1/100 dilution, staining TSPYL1 in a paraffin-embedded NCIN87 Xenograft by Immunohistochemical analysis.

References for Anti-TSPYL1 antibody (ab95943)

ab95943 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Nov 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"