The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPlays a role in regulating placenta growth. May act via its PH domain that competes with other PH domain-containing proteins, thereby preventing their binding to membrane lipids.
Tissue specificityExpressed in placenta and adult prostate gland. In placenta, it is present in all cells of the villous cytotrophoblast. The protein is absent in cells from hydatidiform moles. Hydatidiform mole is a gestation characterized by abnormal development of both fetus and trophoblast. The majority of hydatidiform moles are associated with an excess of paternal to maternal genomes and are likely to result from the abnormal expression of imprinted genes (at protein level). Expressed at low levels in adult liver and lung, and fetal liver. Expressed in adult brain and neuroblastoma, medullablastoma and glioblastoma cell lines.
Sequence similaritiesBelongs to the PHLDA2 family. Contains 1 PH domain.
DomainThe PH domain binds phosphoinositides with a broad specificity. It may compete with the PH domain of some other proteins, thereby interfering with their binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling TSSC3 with ab99209 at 1/100. A Cy3-conjugate donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm and membrane. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TSSC3. Right - Merge.
Western blot - TSSC3 antibody (ab99209)
Anti-TSSC3 antibody (ab99209) at 1 µg/ml + HT1080 cell lysate at 10 µg
Predicted band size : 17 kDa Gel concentration 10-20%
References for Anti-TSSC3 antibody (ab99209)
This product has been referenced in:
Dai H et al. TSSC3 overexpression associates with growth inhibition, apoptosis induction and enhances chemotherapeutic effects in human osteosarcoma. Carcinogenesis : (2011).
Read more (PubMed: 22021909) »