Anti-TSSK4 antibody - C-terminal (ab170349)

Overview

  • Product name
    Anti-TSSK4 antibody - C-terminal
    See all TSSK4 primary antibodies
  • Description
    Rabbit polyclonal to TSSK4 - C-terminal
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TSSK4 aa 233-262 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6SA08

  • Positive control
    • 293 cell lysates transiently transfected with the TSSK4, Human testis tissue and 293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170349 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab170349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.

Target

  • Function
    May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.
  • Tissue specificity
    Expressed only in the testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf20 antibody
    • Serine/threonine-protein kinase 22E antibody
    • STK22E antibody
    • Testis-specific kinase 4 antibody
    • Testis-specific serine/threonine-protein kinase 4 antibody
    • TSK-4 antibody
    • TSSK-4 antibody
    • Tssk4 antibody
    • TSSK4_HUMAN antibody
    • TSSK5 antibody
    see all

Images

  • All lanes : Anti-TSSK4 antibody - C-terminal (ab170349) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : 293 cell lysates transfected with TSSK4

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 37 kDa
  • Immunohistochemistry analysis of formalin-fixed paraffin-embedded  Human testis tissue labeling TSSK4 with ab170349 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining .

  • Immunofluorescence analysis of 293 cells labeling TSSK4 with ab170349 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

References

ab170349 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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