Anti-IFT88 antibody (ab42497)
Key features and details
- Goat polyclonal to IFT88
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IFT88 antibody
See all IFT88 primary antibodies -
Description
Goat polyclonal to IFT88 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2). -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog -
Immunogen
Synthetic peptide corresponding to Human IFT88 aa 804-816 (C terminal).
Sequence:C-KKRIDEDDFADEE
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Positive control
- WB: A549 cell lysate and Mouse testis tissue lysate; ICC/IF: HepG2 cells; Flow Cyt (intra): HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Involved in primary cilium biogenesis. -
Tissue specificity
Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 12 TPR repeats. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > cilium basal body. - Information by UniProt
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Database links
- Entrez Gene: 8100 Human
- Entrez Gene: 21821 Mouse
- Entrez Gene: 305918 Rat
- Omim: 600595 Human
- SwissProt: Q13099 Human
- SwissProt: Q61371 Mouse
- Unigene: 187376 Human
- Unigene: 4653 Mouse
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Alternative names
- D13S1056E antibody
- DAF19 antibody
- hTg737 antibody
see all
Images
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All lanes : Anti-IFT88 antibody (ab42497) at 3 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 94 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells labeling IFT88 with ab42497. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor® 488 conjugated secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Flow cytometric analysis of paraformaldehyde fixed HepG2 cells labeling IFT88 with ab42497 (blue line). Cells permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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ab42497 staining IFT88 in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton ×100. Samples were incubated with primary antibody (1/100:in DMEM w/ 10% FBS) for 1 hour at 200C. An Alexa Fluor ® 546-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab42497 has been referenced in 11 publications.
- Aghaizu ND et al. Repeated nuclear translocations underlie photoreceptor positioning and lamination of the outer nuclear layer in the mammalian retina. Cell Rep 36:109461 (2021). PubMed: 34348137
- Yang TT et al. Single-particle tracking localization microscopy reveals nonaxonemal dynamics of intraflagellar transport proteins at the base of mammalian primary cilia. Mol Biol Cell 30:828-837 (2019). PubMed: 30759057
- Gerondopoulos A et al. Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex. Curr Biol 29:3323-3330.e8 (2019). PubMed: 31564489
- Walker RV et al. Ciliary exclusion of Polycystin-2 promotes kidney cystogenesis in an autosomal dominant polycystic kidney disease model. Nat Commun 10:4072 (2019). PubMed: 31492868
- Weng RR et al. Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening. Biophys J 115:263-275 (2018). PubMed: 29866362