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Product name Anti-TTC10 antibody See all TTC10 primary antibodies
Goat polyclonal to TTC10
Specificity This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2).
Tested applications ICC/IF, IHC-P more details
Predicted to work with:
C-KKRIDEDDFADEE , corresponding to C terminal amino acids 804-816 of Human TTC10
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 19470799
Use a concentration of 3 µg/ml.
Western Blot: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Function Involved in primary cilium biogenesis.
Tissue specificity Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
Sequence similarities Contains 12 TPR repeats.
Cellular localization Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > cilium basal body.
Information by UniProt
Anti-TTC10 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TTC10 antibody (ab42497)
ab42497 (3µg/ml) staining of paraffin embedded Human Kidney. The tissue sections were subjected to antigen retrieval by microwave in Tris/EDTA buffer. The HRP-staining procedure was used for detection.
Immunocytochemistry/ Immunofluorescence - TTC10 antibody (ab42497) This image is a courtesy of Luke Hofkamp
ab42497 staining TTC10 in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton ×100. Samples were incubated with primary antibody (1/100:in DMEM w/ 10% FBS) for 1 hour at 20 0C. An Alexa Fluor ® 546-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"