Overview

  • Product nameAnti-TTC10 antibodySee all TTC10 primary antibodies ...
  • Description
    Goat polyclonal to TTC10
  • SpecificityThis antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2).
  • Tested applicationsICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide: C-KKRIDEDDFADEE, corresponding to C terminal amino acids 804-816 of Human TTC10

  • Positive control
    • Human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab42497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent dilution. PubMed: 19470799
IHC-P Use a concentration of 3 µg/ml.
Western Blot: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).

Target

  • FunctionInvolved in primary cilium biogenesis.
  • Tissue specificityExpressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
  • Sequence similaritiesContains 12 TPR repeats.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > cilium basal body.
  • Target information above from: UniProt accession Q13099 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • D13S1056E antibody
    • DAF19 antibody
    • hTg737 antibody
    • IFT88 antibody
    • IFT88_HUMAN antibody
    • Intraflagellar transport 88 homolog antibody
    • Intraflagellar transport protein 88 homolog antibody
    • MGC26259 antibody
    • Polaris homolog antibody
    • Probe hTg737 (polycystic kidney disease, autosomal recessive) antibody
    • Recessive polycystic kidney disease protein Tg737 homolog antibody
    • RP11-172H24.2 antibody
    • Tetratricopeptide repeat domain 10 antibody
    • Tetratricopeptide repeat protein 10 antibody
    • TG737 antibody
    • TPR repeat protein 10 antibody
    • TTC10 antibody
    see all

Anti-TTC10 antibody images

  • ab42497 (3µg/ml) staining of paraffin embedded Human Kidney. The tissue sections were subjected to antigen retrieval by microwave in Tris/EDTA buffer. The HRP-staining procedure was used for detection.

  • ab42497 staining TTC10 in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton ×100. Samples were incubated with primary antibody (1/100:in DMEM w/ 10% FBS) for 1 hour at 200C. An Alexa Fluor ® 546-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.

    See Abreview

References for Anti-TTC10 antibody (ab42497)

This product has been referenced in:
  • Bodle JC  et al. Primary cilia: the chemical antenna regulating human adipose-derived stem cell osteogenesis. PLoS One 8:e62554 (2013). ICC/IF ; Human . Read more (PubMed: 23690943) »
  • Miyoshi K  et al. Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice. FASEB J 23:3289-97 (2009). ICC/IF ; Mouse . Read more (PubMed: 19470799) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate epithelial - primary)
Specification Prostate epithelial - primary
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% triton x-100
Username

Dr. Luke Hofkamp

Verified customer

Submitted Feb 09 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Retrieval
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"