• Product nameAnti-TTC11 antibody
    See all TTC11 primary antibodies
  • Description
    Rabbit polyclonal to TTC11
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-122 of Human TTC11 (NP_057152).

  • Positive control
    • Raji whole cell lysate; NCIN87 xenograft; 293T, HeLa and Molt-4 lysates



Our Abpromise guarantee covers the use of ab96764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/500.


  • FunctionPromotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
  • Sequence similaritiesBelongs to the FIS1 family.
    Contains 1 TPR repeat.
  • DomainThe C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.
  • Post-translational
    Ubiquitinated by MARCH5.
  • Cellular localizationMitochondrion outer membrane. Peroxisome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010003O14Rik antibody
    • CGI 135 antibody
    • CGI135 antibody
    • FIS 1 antibody
    • FIS1 antibody
    • Fis1 homolog antibody
    • FIS1, S. cerevisiae, homolog of antibody
    • FIS1_HUMAN antibody
    • Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody
    • Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody
    • Fission 1 (mitochondrial outer membrane) homolog antibody
    • Fission 1 homolog antibody
    • H NH0132A01.6 antibody
    • hFis 1 antibody
    • hFis1 antibody
    • Mitochondrial fission 1 protein antibody
    • mitochondrial fission molecule antibody
    • Tetratricopeptide repeat domain 11 antibody
    • Tetratricopeptide repeat protein 11 antibody
    • TPR repeat protein 11 antibody
    • TTC 11 antibody
    see all

Anti-TTC11 antibody images

  • Anti-TTC11 antibody (ab96764) at 1/10000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 17 kDa
  • Immunohistochemical analysis of TTC11 in paraffin-embedded NCIN87 xenograft, using ab96764 antibody at 1/100 dilution.
  • ICC/IF image of ab96764 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96764, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TTC11 antibody (ab96764)

This product has been referenced in:
  • Kitaoka Y  et al. Combined effects of resistance training and calorie restriction on mitochondrial fusion and fission proteins in rat skeletal muscle. J Appl Physiol (1985) 121:806-810 (2016). Read more (PubMed: 27539498) »
  • Kitaoka Y  et al. Nrf2 deficiency does not affect denervation-induced alterations in mitochondrial fission and fusion proteins in skeletal muscle. Physiol Rep 4:N/A (2016). WB ; Mouse . Read more (PubMed: 28039408) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293T)
Permeabilization Yes - 0.1% Triton X-100
Specification HEK293T
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde

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Verified customer

Submitted Jul 14 2017

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Jul 15 2014