Anti-TTC11/FIS1 antibody (ab96764)
Key features and details
- Rabbit polyclonal to TTC11/FIS1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TTC11/FIS1 antibody
See all TTC11/FIS1 primary antibodies -
Description
Rabbit polyclonal to TTC11/FIS1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human TTC11/FIS1 aa 1-122.
Database link: NP_057152 -
Positive control
- Raji whole cell lysate; NCIN87 xenograft; 293T, HeLa and Molt-4 lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/100 - 1/500.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. -
Sequence similarities
Belongs to the FIS1 family.
Contains 1 TPR repeat. -
Domain
The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L. -
Post-translational
modificationsUbiquitinated by MARCH5. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. - Information by UniProt
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Database links
- Entrez Gene: 615565 Cow
- Entrez Gene: 51024 Human
- Entrez Gene: 66437 Mouse
- Entrez Gene: 288584 Rat
- Omim: 609003 Human
- SwissProt: Q3T0I5 Cow
- SwissProt: Q9Y3D6 Human
- SwissProt: Q9CQ92 Mouse
see all -
Alternative names
- 2010003O14Rik antibody
- CGI 135 antibody
- CGI135 antibody
see all
Images
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Anti-TTC11/FIS1 antibody (ab96764) at 1/10000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 17 kDa
15% SDS-PAGE -
Immunohistochemical analysis of TTC11/FIS1 in paraffin-embedded NCIN87 xenograft, using ab96764 antibody at 1/100 dilution.
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ICC/IF image of ab96764 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96764, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab96764 has been referenced in 21 publications.
- Shao SM et al. Electroacupuncture Attenuates Learning and Memory Impairment via PI3K/Akt Pathway in an Amyloid β25-35-Induced Alzheimer's Disease Mouse Model. Evid Based Complement Alternat Med 2022:3849441 (2022). PubMed: 35463064
- Harmuth T et al. Mitochondrial Dysfunction in Spinocerebellar Ataxia Type 3 Is Linked to VDAC1 Deubiquitination. Int J Mol Sci 23:N/A (2022). PubMed: 35682609
- Chen W et al. Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect. Int J Nanomedicine 17:5431-5446 (2022). PubMed: 36426375
- Kitaoka Y et al. Voluntary exercise prevents abnormal muscle mitochondrial morphology in cancer cachexia mice. Physiol Rep 9:e15016 (2021). PubMed: 34427401
- Takahashi K et al. Lactate administration does not affect denervation-induced loss of mitochondrial content and muscle mass in mice. FEBS Open Bio 11:2836-2844 (2021). PubMed: 34510821