Recombinant
RabMAb

Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Overview

  • Product name
    Anti-TTC19 antibody [EPR13184] - C-terminal
    See all TTC19 primary antibodies
  • Description
    Rabbit monoclonal [EPR13184] to TTC19 - C-terminal
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TTC19 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6DKK2

  • Positive control
    • HT-1080 cell and Human fetal muscle, fetal heart and ovarian cancer lysates. HT-1080 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13184
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).

The observed MW is lower than the predicted 42kDa, which may be due to the removing of 8KDa transit peptide.

ICC/IF 1/100 - 1/250.
IP 1/70.

Target

  • Function
    Mitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence.
  • Involvement in disease
    Mitochondrial complex III deficiency, nuclear 2
  • Sequence similarities
    Belongs to the TTC19 family.
    Contains 5 TPR repeats.
  • Cellular localization
    Mitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010204O13Rik antibody
    • FLJ20343 antibody
    • MGC138312 antibody
    • MGC19520 antibody
    • mitochondrial antibody
    • RIKEN cDNA 2810460C24 antibody
    • Tetratricopeptide repeat protein 19 antibody
    • TPR repeat protein 19 antibody
    • Ttc 19 antibody
    • TTC19 antibody
    • TTC19_HUMAN antibody
    see all

Images

  • All lanes : Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/2000 dilution

    Lane 1 : Human fetal muscle tissue lysate
    Lane 2 : Human ovarian cancer tissue lysate
    Lane 3 : Human fetal heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Predicted band size : 42 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/10000 dilution + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size : 42 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HT-1080 cells, staining TTC19 (green) with ab181069. Goat anti rabbit IgG (Alexa Fluor®488) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue)

  • Western blot analysis on immunoprecipitation pellet from HT-1080, labeling TTC19 immunoprecipitated using ab181069 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

ab181069 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (MEF & HELA)
Gel Running Conditions
Non-reduced Denaturing (10% mops)
Loading amount
20 µg
Specification
MEF & HELA
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Feb 01 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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