Recombinant Anti-TTC27 antibody [EPR13653] (ab181162)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13653] to TTC27
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TTC27 antibody [EPR13653]
See all TTC27 primary antibodies -
Description
Rabbit monoclonal [EPR13653] to TTC27 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela and A673 cell lysates, Hela cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13653 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Sequence similarities
Belongs to the TTC27 family.
Contains 3 HAT repeats.
Contains 7 TPR repeats. - Information by UniProt
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Database links
- Entrez Gene: 55622 Human
- Entrez Gene: 74196 Mouse
- SwissProt: Q6P3X3 Human
- SwissProt: Q8CD92 Mouse
- Unigene: 468125 Human
- Unigene: 209503 Mouse
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Alternative names
- FLJ20272 antibody
- Tetratricopeptide repeat domain 27 antibody
- Tetratricopeptide repeat protein 27 antibody
see all
Images
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All lanes : Anti-TTC27 antibody [EPR13653] (ab181162) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A673 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa -
Immunocytochemical staining of HeLa cells fixed in Acetone at -20℃, labeling TTC27 with ab181162 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution (red) and counterstained with DAPI (blue)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181162 has not yet been referenced specifically in any publications.