Overview

  • Product name
  • Description
    Rabbit polyclonal to TTC3
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 19-145 of Human TTC3.

  • Positive control
    • Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    E3 ubiquitin-protein ligase that mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus. Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation. Catalyzes the formation of 'Lys-48'-polyubiquitin chains. May play a role in neuronal differentiation inhibition via its interaction with CIT.
  • Tissue specificity
    Found in all tissues examined.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
    Contains 4 TPR repeats.
  • Post-translational
    modifications
    Phosphorylation on Ser-378 by Akt is required for ubiquitin ligase activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCRR1 antibody
    • E3 ubiquitin protein ligase TTC3 antibody
    • E3 ubiquitin-protein ligase TTC3 antibody
    • Protein DCRR1 antibody
    • RING finger protein 105 antibody
    • RNF105 antibody
    • Tetratricopeptide repeat protein 3 (TPR repeat protein D) antibody
    • Tetratricopeptide repeat protein 3 antibody
    • TPR repeat protein 3 antibody
    • TPR repeat protein D antibody
    • TPRD antibody
    • TPRDI antibody
    • TPRDII antibody
    • TPRDIII antibody
    • TTC3 antibody
    • TTC3_HUMAN antibody
    see all

Images

  • ab121783, staining TTC3 in paraffin-embedded Human Liver tissue, by Immunohistochemistry.
  • ab121783, at 4 µg/ml, staining TTC3 (green) in PFA-fixed, Triton-permeabilized Human A-431 cells, by Immunofluorescence.

References

ab121783 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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