Overview

  • Product name
    Anti-TTC31 antibody
  • Description
    Rabbit polyclonal to TTC31
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GEEDIVDFLR RLVESDPQGL HRIHVDGSSG RLQLWHHDYL LGHLDDEGKS TGQSDRGKGA EGLGTYCGLR KSFLYPPQES EPCPQSPSA, corresponding to amino acids 41-129 of Human TTC31.

  • Positive control
    • Human vagina tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.

Target

Images

  • Lane 1 :
    Lane 2 :

    Lane 1 : Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
    Lane 2 : Human Tonsil tissue


    Predicted band size : 57 kDa
  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122745 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122745 at 1/200 dilution staining TTC31 in Paraffin-embedded Human vagina tissue by Immunohistochemistry.

References

ab122745 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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