The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tissue specificityWidely expressed with the highest levels observed in vascular tissues, lymph node, pituitary, lung and intestine. Not expressed in the liver.
Involvement in diseaseNote=Defects in TTC37 are associated with Trico-Hepato-Enteric syndrome (THE). THE syndrome is a rare and severe autosonmal recessive condition that associates intractable diarrhea with facial dysmorphism, intrauterine growth retardation immunodeficiency with low serum concentrations of immunoglobulins and hair abnormalities characterized by woolly hair.