Overview

  • Product nameAnti-TTC4 antibody
    See all TTC4 primary antibodies
  • Description
    Mouse monoclonal to TTC4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Rhesus monkey
  • Immunogen

    GST-tagged recombinant fragment: MEQPGQDPTS DDVMDSFLEK FQSQPYRGGF HEDQWEKEFE KVPLFMTRAP SEIDPRENPD LACLQSIIFD EERSPEEQAK TYKDEGNDYF KEKDYKKAVI, corresponding to N terminal amino acids 1-100 of Human TTC4 (NP_004614)

  • Positive control
    • TTC4-transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceTTC4 contains three tetratricopeptide repeats. The 34-amino acid tetratricopeptide repeat (TPR) motif is found in a variety of proteins and may mediate protein-protein or protein-membrane interactions.
  • Database links
  • Alternative names
    • DKFZp781B0622 antibody
    • FLJ41930 antibody
    • MGC5097 antibody
    • My044 protein antibody
    • OTTHUMP00000011556 antibody
    • Tetratricopeptide repeat domain 4 antibody
    • Tetratricopeptide repeat protein 4 antibody
    • TPR repeat protein 4 antibody
    • TTC 4 antibody
    see all

Anti-TTC4 antibody images

  • Anti-TTC4 antibody (ab77143) at 5 µg/ml + immunogen (GST-tagged 100 aa recombinant fragment) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 45 kDa
  • All lanes : Anti-TTC4 antibody (ab77143) at 5 µg/ml

    Lane 1 : TTC4-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TTC4 antibody (ab77143)

ab77143 has not yet been referenced specifically in any publications.

Product Wall

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Thank you for your email. I am sorry to hear that you are having troubles using ab77143. The protocol looks fine to me however I would like to make few comments/suggestions that might help to improve your results and that you could consider trying: - u...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"