Recombinant
RabMAb

Anti-TTC4 antibody [EPR13381-76] (ab181195)

Overview

  • Product name
    Anti-TTC4 antibody [EPR13381-76]
    See all TTC4 primary antibodies
  • Description
    Rabbit monoclonal [EPR13381-76] to TTC4
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human TTC4 aa 1-150. The exact sequence is proprietary.
    Database link: O95801

  • Positive control
    • 293T, HepG2, MCF7 and U87-MG cell lysates; Human testis and Human transitional cell carcinoma of bladder tissue; MCF7 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13381-76
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF 1/100 - 1/250.

Target

  • Relevance
    TTC4 contains three tetratricopeptide repeats. The 34-amino acid tetratricopeptide repeat (TPR) motif is found in a variety of proteins and may mediate protein-protein or protein-membrane interactions.
  • Database links
  • Alternative names
    • DKFZp781B0622 antibody
    • FLJ41930 antibody
    • MGC5097 antibody
    • My044 protein antibody
    • OTTHUMP00000011556 antibody
    • Tetratricopeptide repeat domain 4 antibody
    • Tetratricopeptide repeat protein 4 antibody
    • TPR repeat protein 4 antibody
    • TTC 4 antibody
    see all

Images

  • All lanes : Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/20000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 45 kDa
  • Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/5000 dilution + MCF7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 45 kDa
  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TTC4 with ab181195 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181195 at 1/250 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at a 1/200 dilution. Conterstained with Dapi (blue).

References

ab181195 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181195.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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