Overview

  • Product nameAnti-TTC8 antibody
    See all TTC8 primary antibodies
  • Description
    Goat polyclonal to TTC8
  • Specificityab101731 is expected to recognize all reported isoforms (NP_653197.2; NP_938051.1; NP_938052.1).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-KEVLKQDNTHVE

    , corresponding to internal sequence amino acids 316-327 of Human TTC8 (NP_653197.2).

  • Positive control
    • Human Testis lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab101731 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 62 kDa).

Target

  • FunctionThe BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in TTC8 are the cause of retinitis pigmentosa type 51 (RP51) [MIM:613464]. It is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in TTC8 are the cause of Bardet-Biedl syndrome type 8 (BBS8) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect.
  • Sequence similaritiesContains 8 TPR repeats.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cell projection > cilium membrane. Cytoplasm. Localizes to nonmembranous centriolar satellites in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bardet Biedl syndrome 8 protein antibody
    • Bardet Biedl syndrome type 8 antibody
    • Bardet-Biedl syndrome 8 protein antibody
    • BBS8 antibody
    • RP51 antibody
    • Tetratricopeptide repeat domain 8 antibody
    • Tetratricopeptide repeat protein 8 antibody
    • TPR repeat protein 8 antibody
    • TTC 8 antibody
    • Ttc8 antibody
    • TTC8_HUMAN antibody
    see all

Anti-TTC8 antibody images

  • Anti-TTC8 antibody (ab101731) at 0.2 µg/ml + Human testis lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 62 kDa

References for Anti-TTC8 antibody (ab101731)

ab101731 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"