The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 8 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceTTDA, the tenth subunit of the basal transcription factor TFIIH, has a critical role in DNA nucleotide excision repair, where it triggers DNA opening by stimulating XPB ATPase activity together with the damage recognition factor XPC. Defects in TTDA are a cause of trichothiodystrophy