Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP1584Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 38-42 kDa (predicted molecular weight: 38-42 kDa).Can be blocked with TTF1 peptide (ab187893).
Flow Cyt 1/20 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/400.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionTranscription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
    • Tissue specificityThyroid and lung.
    • Involvement in diseaseDefects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
      Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
    • Sequence similaritiesBelongs to the NK-2 homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AV026640 antibody
      • BCH antibody
      • Benign chorea antibody
      • BHC antibody
      • Homeobox protein NK 2 homolog A antibody
      • Homeobox protein NK-2 homolog A antibody
      • Homeobox protein Nkx 2.1 antibody
      • Homeobox protein Nkx-2.1 antibody
      • Homeobox protein Nkx2.1 antibody
      • NK 2 antibody
      • NK 2 homolog A antibody
      • NK2 antibody
      • NK2 homeobox 1 antibody
      • NK2, drosophila, homolog of, A antibody
      • NK2.1, mouse, homolog of antibody
      • Nkx 2 1 antibody
      • NKX 2.1 antibody
      • NKX 2A antibody
      • NKX2 1 antibody
      • Nkx2-1 antibody
      • NKX2.1 antibody
      • NKX21_HUMAN antibody
      • NKX2A antibody
      • T EBP antibody
      • T/EBP antibody
      • TEBP antibody
      • Thyroid nuclear factor 1 antibody
      • Thyroid nuclear factor antibody
      • Thyroid specific enhancer binding protein antibody
      • Thyroid transcription factor 1 antibody
      • Tin man antibody
      • Tinman antibody
      • TITF 1 antibody
      • TITF1 antibody
      • TTF 1 antibody
      • TTF-1 antibody
      • TTF1 antibody
      see all

    Anti-TTF1 antibody [EP1584Y] images

    • Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution + HEK293 whole cell lysate at 30 µg

      Secondary
      HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 38-42 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes

      This image is courtesy of an anonymous Abreview

      Blocked with 5% milk

      See Abreview

    • Immunofluorescence analysis of rat bronchi cells, staining TTF1 with ab76013.

      Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 16°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

      See Abreview

    • ab76013 staining TTF1 in murine fetal lung tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in formaldehyde and permeabilized using PBST. Samples were then blocked using 1.5% serum for 20 minutes at 25°C, then incubated with ab76013 at a 1/400 dilution for 12 hours at 4°C. A biotin conjugated goat anti-rabbit IgG was used as the secondary at a 1/200 dilution.

      See Abreview

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling TTF1 with ab76013 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • All lanes : Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Mouse lung lysate
      Lane 3 : Rat lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 38-42 kDa
      Observed band size : 36,39 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing A549 cells stained with ab76013 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76013, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    References for Anti-TTF1 antibody [EP1584Y] (ab76013)

    This product has been referenced in:
    • Torigoe M  et al. Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium. Sci Rep 5:12258 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 26193445) »
    • Tyson JA  et al. Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells. Development 142:1267-78 (2015). Read more (PubMed: 25804737) »

    See all 19 Publications for this product

    Product Wall

    Application Flow Cytometry
    Sample Human Cell (Differentiated hNSCs)
    Permeabilization Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy Undifferentiated Stem Cells (white)
    Specification Differentiated hNSCs
    Preparation Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Human Cell (Differentiated hNSCs)
    Specification Differentiated hNSCs
    Permeabilization Yes - 0.25% Triton X-100 in DPBS
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 13 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (MLE12 cells)
    Loading amount 20 µg
    Specification MLE12 cells
    Treatment TTF-1 Plasmid (2ug)
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 20 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Mouse bronchiolar epithelium)
    Specification Mouse bronchiolar epithelium
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 23 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Rat Bronchi)
    Specification Rat Bronchi
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Frozen sections of human lung parenchyme)
    Specification Frozen sections of human lung parenchyme
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Whole Lung)
    Loading amount 30 µg
    Specification Whole Lung
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 05 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293)
    Loading amount 30 µg
    Specification HEK293
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 05 2012

    For an anti-rabbit IgG secondary conjugated to APC (Allophycocyanin), we recommend ab130805:

    Click here (or use the following: http://www.abcam.com/index.html?datasheet=130805).

    Please let me know if you have any questions.

    Thank you for your reply. Your protocol looks correct. The only possible issues I see are the concentration of the secondary antibody that you incubate with the cells but more importantly, the lack of saponin in the wash buffer. The saponin permeabiliz...

    Read More

    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"