Overview

  • Product name
  • Description
    Rabbit polyclonal to TTF2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 225-275 of Human TTF2 (NP_003585.3).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab112078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 130 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • F2 antibody
    • Factor 2 antibody
    • Factor2 antibody
    • HuF 2 antibody
    • HuF2 antibody
    • Human factor 2 antibody
    • Lodestar homolog antibody
    • Lodestar protein antibody
    • RNA polymerase II termination factor antibody
    • Transcription release factor 2 antibody
    • Transcription termination factor 2 antibody
    • Transcription termination factor RNA polymerase II antibody
    • TTF2 antibody
    • TTF2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TTF2 antibody (ab112078) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 130 kDa


    Exposure time : 3 minutes
  • Detection of TTF2 by Western Blot of Immunprecipitate.
    ab112078 at 1µg/ml staining TTF2 in HeLa whole cell lysate immunoprecipitated using ab112078 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab112078 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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