FunctionDsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.
Transcription termination factor RNA polymerase II antibody
Anti-TTF2 antibody images
Western blot - TTF2 antibody (ab112078)
All lanes : Anti-TTF2 antibody (ab112078) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 130 kDa
Exposure time : 3 minutes
Immunoprecipitation - TTF2 antibody (ab112078)
Detection of TTF2 by Western Blot of Immunprecipitate.
ab112078 at 1µg/ml staining TTF2 in HeLa whole cell lysate immunoprecipitated using ab112078 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-TTF2 antibody (ab112078)
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