Overview

  • Product name
  • Description
    Rabbit polyclonal to TTF2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 232-511 of Human TTF2 (UniProt ID: Q9UNY4).

  • Positive control
    • H1299, HepG2, Raji, 293T and A431 whole cell lysates; HeLa cell; A549 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • F2 antibody
    • Factor 2 antibody
    • Factor2 antibody
    • HuF 2 antibody
    • HuF2 antibody
    • Human factor 2 antibody
    • Lodestar homolog antibody
    • Lodestar protein antibody
    • RNA polymerase II termination factor antibody
    • Transcription release factor 2 antibody
    • Transcription termination factor 2 antibody
    • Transcription termination factor RNA polymerase II antibody
    • TTF2 antibody
    • TTF2_HUMAN antibody
    see all

Images

  • Anti-TTF2 antibody (ab155177) at 1/5000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 130 kDa
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue, labeling TTF2 with ab155177 at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cell labeling TTF2 with ab155177 at 1/500 dilution. Lower image was costained with Hoechst 33342.

References

ab155177 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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