Overview

  • Product name
  • Description
    Sheep polyclonal to TTF2
  • Host species
    Sheep
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein, heterogeneously sized (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab28030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • F2 antibody
    • Factor 2 antibody
    • Factor2 antibody
    • HuF 2 antibody
    • HuF2 antibody
    • Human factor 2 antibody
    • Lodestar homolog antibody
    • Lodestar protein antibody
    • RNA polymerase II termination factor antibody
    • Transcription release factor 2 antibody
    • Transcription termination factor 2 antibody
    • Transcription termination factor RNA polymerase II antibody
    • TTF2 antibody
    • TTF2_HUMAN antibody
    see all

Images

  • Anti-TTF2 antibody (ab28030) at 1/1000 dilution + HeLa nuclear extract

    Secondary
    rabbit anti-sheep peroxidase linked at 1/10000 dilution

    Predicted band size: 130 kDa
    Observed band size: 150 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Ramakrishnan R  et al. Identification of novel CDK9 and Cyclin T1-associated protein complexes (CCAPs) whose siRNA depletion enhances HIV-1 Tat function. Retrovirology 9:90 (2012). WB . Read more (PubMed: 23110726) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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