Overview

  • Product name
    Anti-TTI1 antibody - N-terminal
    See all TTI1 primary antibodies
  • Description
    Rabbit polyclonal to TTI1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human TTI1 aa 1-50 (N terminal). The exact sequence is proprietary.
    Sequence:

    MAVFDTPEEA FGVLRPVCVQ LTKTQTVENV EHLQTRLQAV SDSALQELQQ


    Database link: O43156

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance
    TTI1 promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals. TTI1 interacts with TELO2, MTOR, ATM, ATR, PRKDC, SMG1 and TRRAP.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • TTI1 antibody
    • KIAA0406 antibody
    • smg 10 antibody
    • smg 10 homolog nonsense mediated mRNA decay factor antibody
    • SMG10 antibody
    • Tel two interacting protein 1 antibody
    • Tel2 interacting protein 1 homolog (S. pombe) antibody
    • TEL2 interacting protein 1 homolog antibody
    • TELO2 interacting protein 1 antibody
    see all

Images

  • All lanes : Anti-TTI1 antibody - N-terminal (ab176696) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 122 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation. ab176696 at 0.4 µg/ml staining TTI1 in HeLa whole cell lysate immunoprecipitated using ab176696 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: control IgG.

References

ab176696 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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