Overview

  • Product nameAnti-TTLL3 antibody
  • Description
    Mouse polyclonal to TTLL3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    TEGDRNIWIV KPGAKSRGRG IMCMDHLEEM LKLVNGNPVV MKDGKWVVQK , corresponding to amino acids 1-102 of Human TTLL3

  • Positive control
    • HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml. Note: requires Protein A purification prior to use in ICC/IF.
    WB: 1/500 - 1/1000. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMonoglycylase which modifies alpha- and beta-tubulin, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues within the C-terminal tail of alpha-and beta-tubulin. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction.
    • Sequence similaritiesContains 1 TTL domain.
    • Cellular localizationCytoplasm > cytoskeleton. Cell projection > cilium. Cytoplasm > cytoskeleton > cilium axoneme.
    • Information by UniProt
    • Database links
    • Alternative names
      • HOTTL antibody
      • PRO0207 antibody
      • Ttll3 antibody
      • TTLL3_HUMAN antibody
      • Tubulin monoglycylase TTLL3 antibody
      • Tubulin tyrosine ligase like protein 3 antibody
      • Tubulin--tyrosine ligase-like protein 3 antibody
      see all

    Anti-TTLL3 antibody images

    • All lanes : Anti-TTLL3 antibody (ab68101) at 1/500 dilution

      Lane 1 : TTLL3 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 87 kDa
      Additional bands at : 10 kDa (possible isoform).
    • Immunofluorescence of purified ab68101 to TTLL3 on HeLa cells. Antibody concentration 10 µg/ml

    References for Anti-TTLL3 antibody (ab68101)

    ab68101 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68101.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"