Polyglutamylase which preferentially modifies beta-tubulin and nucleosome assembly proteins NAP1 and NAP2. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step.
Belongs to the tubulin--tyrosine ligase family. Contains 1 TTL domain.
Cell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Located in cilia. In some cells, also found in basal bodies.
Detection of TTLL4 by Western Blot of Immunprecipitate. Lane 1: ab186045 at 1µg/ml staining TTLL4 in 293T whole cell lysate immunoprecipitated using ab186045 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 3 minutes. Detection: Chemiluminescence with exposure time of 3 minutes.
Western blot - Anti-TTLL4 antibody - C-terminal (ab186045)
All lanes : Anti-TTLL4 antibody - C-terminal (ab186045) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate Lane 2 : 293T whole cell lysate Lane 3 : Jurkat whole cell lysate