The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 133 kDa).
Polyglutamylase which preferentially modifies beta-tubulin and nucleosome assembly proteins NAP1 and NAP2. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step.
Belongs to the tubulin--tyrosine ligase family. Contains 1 TTL domain.
Cell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Located in cilia. In some cells, also found in basal bodies.