The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 65 , 96 kDa).
RelevanceTTLL6 is apolyglutamylase which preferentially modifies alpha-tubulin. It is involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step. TTLL6 contains one TTL domain and two isoforms produced by alternative splicing have been identified.