Overview

  • Product nameAnti-TTLL6 antibody
  • Description
    Mouse polyclonal to TTLL6
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human TTLL6 (Q8N841 isoform 2, 569 aa).

  • Positive control
    • Human colon tissue lysate and TTLL6 transfected 293T lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 65 , 96 kDa).

Target

  • RelevanceTTLL6 is apolyglutamylase which preferentially modifies alpha-tubulin. It is involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step. TTLL6 contains one TTL domain and two isoforms produced by alternative splicing have been identified.
  • Cellular localizationCell projection, cilium. Cell projection, cilium basal body.
  • Database links
  • Alternative names
    • FLJ35808 antibody
    • TTL 6 antibody
    • TTL.6 antibody
    • Tubulin polyglutamylase TTLL6 antibody
    • Tubulin tyrosine ligase like family, member 6 antibody
    see all

Anti-TTLL6 antibody images

  • Anti-TTLL6 antibody (ab69372) at 1/500 dilution + human colon tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 65 , 96 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TTLL6 antibody (ab69372) at 1/500 dilution

    Lane 1 : TTLL6 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 65 , 96 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab69372 at 10µg/ml staining TTLL6 in Hela cells by ICC/IF. 

References for Anti-TTLL6 antibody (ab69372)

ab69372 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"