Anti-TTPA antibody - C-terminal (ab155323)

Overview

  • Product name
    Anti-TTPA antibody - C-terminal
    See all TTPA primary antibodies
  • Description
    Rabbit polyclonal to TTPA - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 215-278 of Human TTPA (UniProt ID: P49638).

  • Positive control
    • IMR32 whole cell lysate; MCF7 cells; Human gastric carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Binds alpha-tocopherol and enhances its transfer between separate membranes.
  • Involvement in disease
    Defects in TTPA are the cause of ataxia with isolated vitamin E deficiency (AVED) [MIM:277460]. AVED is an autosomal recessive disease characterized by spinocerebellar degeneration. It causes ataxia and peripheral neuropathy that resembles Friedreich ataxia. AVED patients have markedly reduced plasma levels of vitamin E.
  • Sequence similarities
    Contains 1 CRAL-TRIO domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-tocopherol transfer protein antibody
    • Alpha-TTP antibody
    • AlphaTTP antibody
    • ATTP antibody
    • AVED antibody
    • Tocopherol (alpha) transfer protein (ataxia (Friedreich-like) with vitamin E deficiency) antibody
    • TTP1 antibody
    • TTPA antibody
    • TTPA_HUMAN antibody
    see all

Images

  • Anti-TTPA antibody - C-terminal (ab155323) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed MCF7 cells labeling TTPA using ab155323 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling TTPA with ab155323 at 1/500 dilution

References

ab155323 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Apr 24 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
40 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Apr 22 2015

Application
Western blot
Sample
Guinea pig Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
40 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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