Overview

  • Product name
    Anti-TUBA1B antibody [EPR1333]
    See all TUBA1B primary antibodies
  • Description
    Rabbit monoclonal [EPR1333] to TUBA1B
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human TUBA1B.

  • Positive control
    • WB: HeLa, Jurkat, 293T, and A431 cell lysates IHC-P: Human placenta and breast tissues ICC/IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR1333
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/500 - 1/1000. Antigen retrieval is recommended.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
    • Sequence similarities
      Belongs to the tubulin family.
    • Post-translational
      modifications
      Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha tubulin ubiquitous antibody
      • Alpha-tubulin ubiquitous antibody
      • K alpha 1 antibody
      • TBA1B_HUMAN antibody
      • TUBA1B antibody
      • Tubulin alpha 1B antibody
      • Tubulin alpha 1B chain antibody
      • Tubulin alpha ubiquitous antibody
      • Tubulin alpha ubiquitous chain antibody
      • Tubulin alpha-1B chain antibody
      • Tubulin alpha-ubiquitous chain antibody
      • Tubulin K alpha 1 antibody
      • Tubulin K-alpha-1 antibody
      see all

    Anti-TUBA1B antibody [EPR1333] images

    • Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) labelling TUBA1B with purified ab108629 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • All lanes : Anti-TUBA1B antibody [EPR1333] (ab108629) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa
      Observed band size : 50 kDa
    • ab108629, at 1/500 dilution, staining TUBA1B in paraffin embedded Human placenta tissue by Immunohistochemistry.
    • ab108629, at a 1/500 dilution, staining TUBA1B in paraffin embedded Human breast tissue by Immunohistochemistry.
    • ab108629, at a 1/250 dilution, staining TUBA1B in HeLa cells by Immunofluorescence.

    References for Anti-TUBA1B antibody [EPR1333] (ab108629)

    This product has been referenced in:
    • Houng JY  et al. Glossogyne tenuifolia (Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase. Chin Med 12:9 (2017). Read more (PubMed: 28400856) »
    • Zheng YC  et al. 1,2,3-Triazole-Dithiocarbamate Hybrids, a Group of Novel Cell Active SIRT1 Inhibitors. Cell Physiol Biochem 38:185-93 (2016). WB ; Human . Read more (PubMed: 26784898) »

    See all 8 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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