• Product nameAnti-TUBA4A antibody
    See all TUBA4A primary antibodies
  • Description
    Rabbit polyclonal to TUBA4A
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey, Zebrafish
  • Immunogen

    21 - 213 of Human TUBA4A (NP_005991).

  • Positive control
    • WB: A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab96743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.


  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin 1 antibody
    • Alpha-tubulin 1 antibody
    • FLJ30169 antibody
    • H2 alpha antibody
    • TBA4A_HUMAN antibody
    • Testis specific alpha tubulin antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA 4A antibody
    • TUBA1 antibody
    • Tuba4a antibody
    • Tubulin alpha 1 (testis specific) antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 4a antibody
    • Tubulin alpha 4A chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin H2-alpha antibody
    see all

Anti-TUBA4A antibody images

  • All lanes : Anti-TUBA4A antibody (ab96743) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 50 kDa
  • ab96743, at a 1/200 dilution, staining TUBA4A in paraformaldehyde fixed HeLa cell by Immunofluorescence analysis.
    Right image is merged with DNA probe.

References for Anti-TUBA4A antibody (ab96743)

ab96743 has not yet been referenced specifically in any publications.

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