Overview

  • Product name
  • Description
    Rabbit polyclonal to TUBA6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 19 - 235 of Human TUBA6 (UniProt ID: Q9BQE3).

  • Positive control
    • NT2D1, IMR32, U87 MG, MCF7 and NIH 3T3 cell lysates; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin 6 antibody
    • Alpha tubulin isotype M alpha 6 antibody
    • Alpha-tubulin 6 antibody
    • bcm948 antibody
    • M[a]6 antibody
    • TBA1C_HUMAN antibody
    • Tuba1c antibody
    • Tubulin alpha 1C chain antibody
    • tubulin alpha 6 antibody
    • Tubulin alpha 6 chain antibody
    • Tubulin alpha-1C chain antibody
    • Tubulin alpha-6 chain antibody
    • tubulin, alpha 1c antibody
    see all

Images

  • Anti-TUBA6 antibody (ab155330) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Anti-TUBA6 antibody (ab155330) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed MCF7 cells labeling TUBA6 using ab155330 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab155330 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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