Overview

  • Product name
  • Description
    Rabbit polyclonal to TUBA8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-271 of Human TUBA8 (UniProt ID: Q9NY65).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and NIH 3T3 cell lysates; A431 cells; SW480 xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Tissue specificity
    Preferentially expressed in heart, skeletal muscle and testis. Expressed at low levels in the developing brain.
  • Involvement in disease
    Defects in TUBA8 are the cause of polymicrogyria with optic nerve hypoplasia (PMGONH) [MIM:613180]. It is a disease characterized by extensive polymicrogyria, optic nerve hypoplasia, severe developmental delay, hypotonia, seizures, a dysplastic or absent corpus callosum and colpocephaly. Polymicrogyria is a malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin 8 antibody
    • Alpha-tubulin 8 antibody
    • TBA8_HUMAN antibody
    • Tuba8 antibody
    • TUBAL2 antibody
    • Tubulin alpha 8 chain antibody
    • Tubulin alpha chain like 2 antibody
    • Tubulin alpha chain-like 2 antibody
    • Tubulin alpha-8 chain antibody
    see all

Images

  • All lanes : Anti-TUBA8 antibody (ab154612) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Anti-TUBA8 antibody (ab154612) at 1/5000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TUBA8 using ab154612 at 1/200 dilution. Right panel costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling TUBA8 using ab154612 at 1/500 dilution.

References

ab154612 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154612.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up