Recombinant
RabMAb

Anti-TUBB2A + TUBB2B antibody [EPR11843] (ab170931)

Overview

  • Product name
    Anti-TUBB2A + TUBB2B antibody [EPR11843]
  • Description
    Rabbit monoclonal [EPR11843] to TUBB2A + TUBB2B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human TUBB2A. The exact sequence is proprietary.
    Database link: Q13885

  • Positive control
    • 293T, HepG2, Jurkat and HeLa cell lysates; Human brain tissue; Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC.
  • Target

    Images

    • All lanes : Anti-TUBB2A + TUBB2B antibody [EPR11843] (ab170931) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 50 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TUBB2A with ab170931 at 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from 293T cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170931 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      Lane 1 Primary: ab170931, 1/10000 dilution
      Sample: Immunoprecipitation pellet from 293T cell lysate
      Secondary: HRP-conjugated anti-rabbit IgG

       

       

      Lane 2 Primary: ab170931, 1/10000 dilution
      Sample: 1X PBS (negative control)
      Secondary: HRP-conjugated anti-rabbit IgG

       

    • Flow cytometric analysis of permeabilized Jurkat cells labeling TUBB2A with ab170931 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab170931 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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