Anti-TUBB2A + TUBB2B antibody (ab155311)
Key features and details
- Rabbit polyclonal to TUBB2A + TUBB2B
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-TUBB2A + TUBB2B antibody -
Description
Rabbit polyclonal to TUBB2A + TUBB2B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Monkey -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-237 of Human TUBB2A (UniProt Q13885).
-
Positive control
- 293T, A431, Jurkat, Raji, NIH-3T3 and mouse brain lysates; HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
-
Cellular localization
TUBB2A: Cytoplasm > cytoskeleton. TUBB2B: Cytoplasm, cytoskeleton. -
Database links
- Entrez Gene: 347733 Human
- Entrez Gene: 7280 Human
- Entrez Gene: 22151 Mouse
- Entrez Gene: 73710 Mouse
- Entrez Gene: 291081 Rat
- Entrez Gene: 498736 Rat
- Omim: 612850 Human
- SwissProt: P09203 Chicken
see all
Images
-
Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/3000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling beta II Tubulin with ab155311 at 1/200 dilution. Lower panel co-stained with Hoechst 33342 .
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab155311 has not yet been referenced specifically in any publications.