Overview

  • Product name
  • Description
    Rabbit polyclonal to TUBB2A
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-237 of Human TUBB2A (UniProt Q13885).

  • Positive control
    • 293T, A431, Jurkat, Raji, NIH-3T3 and mouse brain lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Class IIa beta tubulin antibody
    • dJ40E16.7 antibody
    • M(beta)2 antibody
    • TBB2A_HUMAN antibody
    • TUBB antibody
    • TUBB2 antibody
    • Tubb2a antibody
    • Tubulin beta 2 antibody
    • Tubulin beta 2A antibody
    • Tubulin beta 2A class IIa antibody
    • Tubulin beta polypeptide 2 antibody
    • Tubulin beta polypeptide antibody
    • Tubulin beta-2A chain antibody
    see all

Images

  • Anti-TUBB2A antibody (ab155311) at 1/3000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • Anti-TUBB2A antibody (ab155311) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 50 kDa
  • Anti-TUBB2A antibody (ab155311) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling beta II Tubulin with ab155311 at 1/200 dilution. Lower panel co-stained with Hoechst 33342 .

References

ab155311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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