Overview

  • Product name
  • Description
    Rabbit polyclonal to TUBB6
  • Specificity
    The peptide used as the immunogen for the antibody is from Human TUBB6. The immunogen sequence suggests this antibody may cross-react with TUBB 1-5, although we have not tested this.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide between 146-174 amino acids from the central region of Human TUBB6 (NP_115914.1).

  • Positive control
    • K562 cell line lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities
    Belongs to the tubulin family.
  • Domain
    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • tubulin beta-6 chain-like antibody
    • class V beta-tubulin antibody
    • HsT1601 antibody
    • TBB6_HUMAN antibody
    • TUBB-5 antibody
    • TUBB6 antibody
    • Tubulin beta 6 chain antibody
    • tubulin beta 6 class V antibody
    • tubulin beta class V antibody
    • tubulin beta MGC4083 antibody
    • Tubulin beta-6 antibody
    • Tubulin beta-6 chain antibody
    see all

Images

  • Anti-TUBB6 antibody (ab110592) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 50 kDa

References

ab110592 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab110592.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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