Overview

  • Product nameAnti-TUBB8 antibody
    See all TUBB8 primary antibodies
  • Description
    Rabbit polyclonal to TUBB8
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 366 and 430 of Human TUBB8 (NP_817124).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lines

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab97880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA631M21.2 antibody
    • Class VIII beta tubulin antibody
    • HSA10p15 beta tubulin 4Q antibody
    • RP11-631M21.2 antibody
    • TBB8_HUMAN antibody
    • TUBB8 antibody
    • Tubulin beta 8 antibody
    • Tubulin beta 8 chain antibody
    • Tubulin beta 8 class VIII antibody
    • Tubulin beta-8 chain antibody
    see all

Anti-TUBB8 antibody images

  • All lanes : Anti-TUBB8 antibody (ab97880) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
  • Immunofluorescence analysis of TUBB8 in paraformaldehyde fixed HeLa, using ab97880 at a 1/200 dilution.
    Lower image: merged with DNA probe.

References for Anti-TUBB8 antibody (ab97880)

ab97880 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"