Overview

  • Product nameAnti-Tuberin antibody [Y320]
    See all Tuberin primary antibodies
  • Description
    Rabbit monoclonal [Y320] to Tuberin
  • Specificityab32554 recognises Tuberous sclerosis complexe-2 (TCS2 also known as Tuberin. It is predicted to detect isoform 2-4 based on sequence homology.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-term of human TSC2.

  • Positive control
    • WB: Jurkat cell lysate IHC: prostate carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab32554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 200 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • FunctionIn complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
    • Tissue specificityLiver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
    • Involvement in diseaseDefects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
      Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.
    • Sequence similaritiesContains 1 Rap-GAP domain.
    • Post-translational
      modifications
      Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
      Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
    • Cellular localizationCytoplasm. Membrane. At steady state found in association with membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ43106 antibody
      • LAM antibody
      • OTTHUMP00000158940 antibody
      • OTTHUMP00000198394 antibody
      • OTTHUMP00000198395 antibody
      • PPP1R160 antibody
      • Protein phosphatase 1, regulatory subunit 160 antibody
      • tsc2 antibody
      • TSC2_HUMAN antibody
      • TSC4 antibody
      • TSC4 gene, formerly antibody
      • TSC4, formerly antibody
      • Tuberin antibody
      • Tuberous sclerosis 2 antibody
      • Tuberous sclerosis 2 protein antibody
      • Tuberous sclerosis 2 protein homolog antibody
      see all

    Anti-Tuberin antibody [Y320] images

    • Anti-Tuberin antibody [Y320] (ab32554) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 200 kDa
      Observed band size : 220 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing HepG2 cells stained with ab32554 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32554, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References for Anti-Tuberin antibody [Y320] (ab32554)

    This product has been referenced in:
    • Parkhitko AA  et al. Autophagy-dependent metabolic reprogramming sensitizes TSC2-deficient cells to the antimetabolite 6-aminonicotinamide. Mol Cancer Res 12:48-57 (2014). Read more (PubMed: 24296756) »
    • Zhang L  et al. TSC1/2 signaling complex is essential for peripheral naïve CD8+ T cell survival and homeostasis in mice. PLoS One 7:e30592 (2012). WB . Read more (PubMed: 22363451) »

    See all 7 Publications for this product

    Product Wall

    All three antibodies will detect the proteins with or without phosphorylation. The one exception may be the TSC2 antibody, which is raised against a C terminus peptide that contains a phosphorylation site at serine 1798. It is possible that if this ami...

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    Muchas gracias por contactarnos.

    Anticuerpos contra HAMARTINA, o TSC1 testados en IHC-P, tenemos cuatro, dos de conejo policlonal, uno monoclonal también de conejo, y uno policlonal de cabra. En función de las especies en las qu...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 20 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. jp lu

    Verified customer

    Submitted Nov 21 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"