Recombinant Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8202] to Tuberin (phospho S664)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Tuberin (phospho S664) antibody [EPR8202]
See all Tuberin primary antibodies -
Description
Rabbit monoclonal [EPR8202] to Tuberin (phospho S664) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y cell lysates treated with Okadaic acid and Calyculin A and HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled, Human, mouse and rat brain tissue lysate unboiled
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.12 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8202 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 201 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 201 kDa. |
Target
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Function
In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. -
Tissue specificity
Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta. -
Involvement in disease
Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex. -
Sequence similarities
Contains 1 Rap-GAP domain. -
Post-translational
modificationsPhosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. -
Cellular localization
Cytoplasm. Membrane. At steady state found in association with membranes. - Information by UniProt
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Database links
- Entrez Gene: 7249 Human
- Entrez Gene: 22084 Mouse
- Entrez Gene: 24855 Rat
- Omim: 191092 Human
- SwissProt: P49815 Human
- SwissProt: Q61037 Mouse
- SwissProt: P49816 Rat
- Unigene: 90303 Human
see all -
Alternative names
- FLJ43106 antibody
- LAM antibody
- OTTHUMP00000158940 antibody
see all
Images
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All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : Mouse brain lysate boiled
Lane 2 : Mouse brain lysate unboiled
Lane 3 : Rat brain lysate boiled
Lane 4 : Rat brain lysate unboiled
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 201 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
We recommend not to boil the samples after lysis to get desired WB results.
The molecular weight observed is consistent with what has been described in the literature (PMID:30774414; 10335945).
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All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : Human brain lysate unboiled
Lane 2 : Human brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 3 : Rat brain lysate unboiled
Lane 4 : Rat brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 5 : Mouse brain lysate unboiled
Lane 6 : Mouse brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 201 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration 5% NFDM/TBST
Exposure time:
Lane 1, 2: 180 seconds
Lane 3, 4: 40 seconds
Lane 5, 6: 20 seconds -
All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled
Lane 2 : HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled
Lane 3 : HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 201 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 80 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
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All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : SH SY5Y cell lysates
Lane 2 : SH SY5Y cell lysates treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 201 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab133465 has been referenced in 5 publications.
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
- Plescher M et al. TSC2 mediates hyperosmotic stress-induced inactivation of mTORC1. Sci Rep 5:13828 (2015). PubMed: 26345496
- Zhang Y et al. PP2AC Level Determines Differential Programming of p38-TSC-mTOR Signaling and Therapeutic Response to p38-Targeted Therapy in Colorectal Cancer. EBioMedicine 2:1944-56 (2015). PubMed: 26844273
- Peruchetti DB et al. Mis-regulation of Mammalian Target of Rapamycin (mTOR) Complexes Induced by Albuminuria in Proximal Tubules. J Biol Chem 289:16790-801 (2014). WB . PubMed: 24790108
- Jeansonne D et al. Differential Effects of MicroRNAs on Glioblastoma Growth and Migration. Genes (Basel) 4:46-64 (2013). WB . PubMed: 24705102