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Recombinant full length protein, corresponding to amino acids 1-452 of Human Tubulin
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab6161 as a replacement.
Our Abpromise guarantee covers the use of ab56676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use a concentration of 3 µg/ml.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ab56676 at 1/500 dilution staining Tubulin in Human Osteosarcoma cells by immunocytochemistry/ Immunofluorescence. The cells were PFA fixed, permeabilized in PBS (0.5% Triton) and blocked in 1% BSA for 1 hour at 25°C. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG at 1/250 dilution, was used as secondary antibody. The primary antibody shows green staining in image.
This image is courtesy of an Abreview submitted by Xun AiBlocking Step : 5% BSA for 2 hours at 23°C
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"