Recombinant
RabMAb

Anti-Tubulin antibody [EPR13797] - Loading Control (ab185224)

Overview

  • Product name
    Anti-Tubulin antibody [EPR13797] - Loading Control
    See all Tubulin primary antibodies
  • Description
    Rabbit monoclonal [EPR13797] to Tubulin - Loading Control
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Saccharomyces cerevisiae Tubulin aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P09733

  • Positive control
    • Saccharomyces cerevisiae lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab185224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

Target

  • Relevance
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • Tubulin alpha-1 chain antibody
      • Tubulin alpha-3 chain antibody

    Images

    • Anti-Tubulin antibody [EPR13797] - Loading Control (ab185224) at 1/5000 dilution + Saccharomyces cerevisiae lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 50 kDa

    References

    ab185224 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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