The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the TPPP family.
Post-translational modificationsPoor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.
ab105033 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoskeletal-like staining throughout the fibre network.
Western blot - Tubulin Polymerization Promoting Protein antibody (ab105033)
Anti-Tubulin Polymerization Promoting Protein antibody (ab105033) at 0.001 µg/ml + Human Cerebellum lysate at 35 µg Developed using the ECL technique