Anti-Tubulin Polymerization Promoting Protein antibody (ab105033)

Overview

  • Product name
    Anti-Tubulin Polymerization Promoting Protein antibody
    See all Tubulin Polymerization Promoting Protein primary antibodies
  • Description
    Goat polyclonal to Tubulin Polymerization Promoting Protein
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Peptide with sequence

    C-DPSKDRAAKRLSLES

    , corresponding to internal sequence amino acids 21-35 of Human Tubulin Polymerization Promoting Protein (NP_008961.1).

  • Positive control
    • Human Brain (Cerebellum), Mouse and Rat Brain lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the TPPP family.
  • Post-translational
    modifications
    Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
  • Cellular localization
    Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 25 kDa brain specific protein antibody
    • 25 kDa brain-specific protein antibody
    • Brain specific protein p25 alpha antibody
    • Glycogen synthase kinase 3 (GSK3) inhibitor p24 antibody
    • OTTHUMP00000161630 antibody
    • p24 antibody
    • p25 antibody
    • p25-alpha antibody
    • p25alpha antibody
    • TPPP antibody
    • TPPP/p25 antibody
    • TPPP_HUMAN antibody
    • TPPP1 antibody
    • Tubulin polymerization promoting protein antibody
    • Tubulin polymerization-promoting protein antibody
    see all

Images

  • Anti-Tubulin Polymerization Promoting Protein antibody (ab105033) at 0.001 µg/ml + Human Cerebellum lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • ab105033 (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

     

  • ab105033 negative control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

  • ab105033 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoskeletal-like staining throughout the fibre network.

References

ab105033 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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