Recombinant
RabMAb

Anti-Tubulin Polymerization Promoting Protein antibody [EPR3315] (ab119987)

Overview

  • Product name
    Anti-Tubulin Polymerization Promoting Protein antibody [EPR3315]
    See all Tubulin Polymerization Promoting Protein primary antibodies
  • Description
    Rabbit monoclonal [EPR3315] to Tubulin Polymerization Promoting Protein
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Tubulin Polymerization Promoting Protein.

  • Positive control
    • Human fetal brain and SH-SY5Y cell lysates; Human astrocytoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR3315
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the TPPP family.
    • Post-translational
      modifications
      Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
    • Cellular localization
      Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.
    • Information by UniProt
    • Database links
    • Alternative names
      • 25 kDa brain specific protein antibody
      • 25 kDa brain-specific protein antibody
      • Brain specific protein p25 alpha antibody
      • Glycogen synthase kinase 3 (GSK3) inhibitor p24 antibody
      • OTTHUMP00000161630 antibody
      • p24 antibody
      • p25 antibody
      • p25-alpha antibody
      • p25alpha antibody
      • TPPP antibody
      • TPPP/p25 antibody
      • TPPP_HUMAN antibody
      • TPPP1 antibody
      • Tubulin polymerization promoting protein antibody
      • Tubulin polymerization-promoting protein antibody
      see all

    Images

    • All lanes : Anti-Tubulin Polymerization Promoting Protein antibody [EPR3315] (ab119987) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 23 kDa
    • ab119987 at 1/100 dilution staining Tubulin Polymerization Promoting Protein in Paraffin-embedded Human astrocytoma tissue by Immunohistochemistry.

    References

    ab119987 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab119987.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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