The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
1/100 - 1/250. Antigen retrieval is recommended.
Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
1/10 - 1/100.
Application notesIs unsuitable for Flow Cyt or ICC.
FunctionMay play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the TPPP family.
Post-translational modificationsPoor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.