The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionTethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
Tissue specificityUbiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
Involvement in diseaseNote=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein. Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).
Detection of TUG in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab114980 at 3 µg/mg lysate for IP. For WB detection an anti-TUG antibody which recognizes a downstream epitope was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-TUG antibody (ab114980)
has not yet been referenced specifically in any publications.
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