Anti-TUP1 antibody [Clone 10] (ab24313)

Overview

  • Product nameAnti-TUP1 antibody [Clone 10]
  • Description
    Mouse monoclonal [Clone 10] to TUP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-200 of Saccharomyces cerevisiae TUP1

  • Positive control
    • Mav108 cells expressing recombinant TUP1

Properties

Applications

Our Abpromise guarantee covers the use of ab24313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).

Target

  • RelevanceYeast TUP1 Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type specific genes regulated by the alpha2 and a1/alpha2 repressors. Ssn6 Tupl interacts with class I histone deacetylases required for repression, and recruitment of yeast Tuplp Ssn6p repressor is associated with localised decreases in histone acetylation.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • FLK1 antibody
      • Flocculation suppressor protein antibody
      • Repressor AER2 antibody
      • SFL2 antibody
      • Tup1p antibody
      • UMR7 antibody
      see all

    Anti-TUP1 antibody [Clone 10] images

    • All lanes : Anti-TUP1 antibody [Clone 10] (ab24313)

      Lane 1 : Saccharomyces cerevisiae whole cell lysates
      Lane 2 : Saccharomyces cerevisiae whole cell lysates
      Lane 3 : Saccharomyces cerevisiae whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Sheep anti-Mouse polyclonal HRP conjugate at 100 %
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 78 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Image is courtesy of an anonymous AbReview.

      See Abreview

    References for Anti-TUP1 antibody [Clone 10] (ab24313)

    ab24313 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (whole cell)
    Gel Running Conditions Reduced Denaturing
    Loading amount 15 µg
    Specification whole cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 30 2015

    Thank you for your enquiry. The cell line used was transfected to express a fragment of the TUP1 protein, which was the antigen used to develop the antibody. This protein fragment has a molecular weight of approximately 35 kDa. Therefore, the 35k...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"