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Goat polyclonal to TUSC3
Expected to recognise both reported isoforms of TUSC3 (NP_006756.2; NP_839952.1).
Predicted to work with:
Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey
, corresponding to internal sequence amino acids 95-106 of Human TUSC3 isoform a and b (NP_006756.2; NP_839952.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/1000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
TUSC3 belongs to the OST3/OST6 family of proteins and may be involved in N-glycosylation through its association with N-oligosaccharyl transferase.
The TUSC3 gene is a candidate tumour supressor.
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Western blot - TUSC3 antibody (ab77600)
Anti-TUSC3 antibody (ab77600) at 1 µg/ml + Human liver lysate at 35 µg
Predicted band size:
Observed band size:
37 kDa (
why is the actual band size different from the predicted?
Additional bands at:
22 kDa. We are unsure as to the identity of these extra bands.
Both bands were successfully blocked by incubation with the immunising peptide.
This product has been referenced in:
et al. MicroRNA-132 induces temozolomide resistance and promotes the formation of cancer stem cell phenotypes by targeting tumor suppressor candidate 3 in glioblastoma. Int J Mol Med 40:1307-1314 (2017).
Read more (PubMed: 28901390) »
et al. TUSC3 induces autophagy in human non-small cell lung cancer cells through Wnt/ß-catenin signaling. Oncotarget 8:52960-52974 (2017).
Read more (PubMed: 28881786) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"