Overview

  • Product name
  • Description
    Rabbit polyclonal to TUT1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 215-264 (DLDLFLDLGD LEEPQPVPKA PESPSLDSAL ASPLDPQALA CTPASPPDSQ) of human TUT1 (NP_073741).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the DNA polymerase type-B-like family.
    Contains 1 C2H2-type zinc finger.
    Contains 1 PAP-associated domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated by CK1 in the proline-rich (Pro-rich) region. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha regulated poly(A) polymerase antibody
    • nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I antibody
    • nuclear speckle targeted PIPK1A regulated poly(A) polymerase antibody
    • PAP associated domain containing 2 antibody
    • PAPD2 antibody
    • poly(A) polymerase associated domain containing 2 antibody
    • RBM21 antibody
    • RNA binding motif protein 21 antibody
    • RNA-binding motif protein 21 antibody
    • RNA-binding protein 21 antibody
    • Speckle targeted PIP5K1A-regulated poly(A) polymerase antibody
    • Star-PAP antibody
    • STARPAP antibody
    • STPAP_HUMAN antibody
    • terminal uridylyl transferase 1, U6 snRNA specific antibody
    • TUT1 antibody
    • TUTase antibody
    • U6 snRNA specific terminal uridylyltransferase 1 antibody
    • U6 snRNA-specific terminal uridylyltransferase 1 antibody
    • U6 TUTase antibody
    • U6-TUTase antibody
    • URLC6 antibody
    see all

Images

  • Anti-TUT1 antibody (ab86161) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa
    Observed band size : 94 kDa
    Additional bands at : 45 kDa,70 kDa,>168 kDa. We are unsure as to the identity of these extra bands.

References

ab86161 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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