Overview

  • Product nameAnti-TWEAK antibody
    See all TWEAK primary antibodies
  • Description
    Rabbit polyclonal to TWEAK
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length TWEAK (Human)

  • Positive control
    • HT29 (Human colon adenocarcinoma grade II cell line) whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab37170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa). TWEAK may migrate at aberrant poitions in SDS-PAGE.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionBinds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
  • Tissue specificityHighly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APO 3 ligand antibody
    • APO 3L antibody
    • APO3 ligand antibody
    • APO3/DR3 ligand antibody
    • APO3L antibody
    • DR3LG antibody
    • MGC129581 antibody
    • MGC20669 antibody
    • secreted form antibody
    • TNF-related weak inducer of apoptosis antibody
    • TNF12_HUMAN antibody
    • TNFSF 12 antibody
    • Tnfsf12 antibody
    • TNFSF12 protein antibody
    • Tumor necrosis factor (ligand) superfamily member 12 antibody
    • Tumor necrosis factor ligand superfamily member 12 antibody
    • Tumor necrosis factor superfamily member 12 antibody
    • TWEAK antibody
    • UNQ181/PRO207 antibody
    see all

Anti-TWEAK antibody images

  • All lanes : Anti-TWEAK antibody (ab37170) at 1 µg/ml

    Lane 1 : Recombinant human TWEAK at 0.005 µg
    Lane 2 : Recombinant human TWEAK at 0.025 µg
    Lane 3 : Recombinant human TWEAK at 0.05 µg


    Predicted band size : 27 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    TWEAK migrates at aberrant positions in SDS-PAGE; hence the observed band is different from the predicted molecular weight of 27 kDa.
  • ICC/IF image of ab37170 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37170, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TWEAK antibody (ab37170)

This product has been referenced in:
  • Padrão AI  et al. Endurance training prevents TWEAK but not myostatin-mediated cardiac remodelling in cancer cachexia. Arch Biochem Biophys 567:13-21 (2015). Read more (PubMed: 25575785) »
  • Novoyatleva T  et al. FGF1-mediated cardiomyocyte cell cycle reentry depends on the interaction of FGFR-1 and Fn14. FASEB J 28:2492-503 (2014). WB ; Rat . Read more (PubMed: 24571920) »

See all 3 Publications for this product

Product Wall

Thank you for sending along the western blot images. I just have a few additional questions: 1. Are the samples of TWEAK transfected cell extracts or are these samples purified recombinant TWEAK protein or are they untransfected cell lysa...

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