Overview

  • Product nameAnti-TWEAK antibody
    See all TWEAK primary antibodies
  • Description
    Rabbit polyclonal to TWEAK
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse
  • Immunogen

    Synthetic peptide within (GLLLAVVSLG SRASLSAQEP AQEELVAEED QDPSELNPQT EESQDPAPFL) corresponding to N terminal amino acids 36-85 of human TWEAK (NP_003800).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionBinds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
  • Tissue specificityHighly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APO 3 ligand antibody
    • APO 3L antibody
    • APO3 ligand antibody
    • APO3/DR3 ligand antibody
    • APO3L antibody
    • DR3LG antibody
    • MGC129581 antibody
    • MGC20669 antibody
    • secreted form antibody
    • TNF-related weak inducer of apoptosis antibody
    • TNF12_HUMAN antibody
    • TNFSF 12 antibody
    • Tnfsf12 antibody
    • TNFSF12 protein antibody
    • Tumor necrosis factor (ligand) superfamily member 12 antibody
    • Tumor necrosis factor ligand superfamily member 12 antibody
    • Tumor necrosis factor superfamily member 12 antibody
    • TWEAK antibody
    • UNQ181/PRO207 antibody
    see all

Anti-TWEAK antibody images

  • Anti-TWEAK antibody (ab86287) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 27 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TWEAK antibody (ab86287)

ab86287 has not yet been referenced specifically in any publications.

Product Wall

Thank you for sending along the western blot images. I just have a few additional questions: 1. Are the samples of TWEAK transfected cell extracts or are these samples purified recombinant TWEAK protein or are they untransfected cell lysa...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"