The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125µg for 105-8 cells. A 100 µl volume is recommended for staining the cell sample.
ab18403-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionBinds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
Tissue specificityHighly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsThe soluble form derives from the membrane form by proteolytic processing.
Staining of non-transfected (left) and TWEAK transfected (right) L5178Y cells with 0.06 µg of Rat IgG1 Isotype Control Biotin (open histogram) or 0.03 µg of ab95626 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
References for Anti-TWEAK antibody [MTW-1] (Biotin) (ab95626)
This product has been referenced in:
Nakayama M et al. Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies. Biochem Biophys Res Commun306:819-25 (2003).
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