• Product name
  • Description
    Rabbit polyclonal to TWEAKR
  • Specificity
    This antibody reacts with TWEAKR
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human IgG1 Fc-tagged TWEAKR protein

  • Positive control
    • HepG2 cell lysate, 293 cells and human liver tissue



Our Abpromise guarantee covers the use of ab62496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 14 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.


  • Function
    Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • Tissue specificity
    Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • Sequence similarities
    Contains 1 TNFR-Cys repeat.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 266 antibody
    • CD266 antibody
    • CD266 antigen antibody
    • FGF inducible 14 antibody
    • FGF-inducible 14 antibody
    • Fibroblast growth factor inducible immediate early response protein 14 antibody
    • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
    • FN 14 antibody
    • FN14 antibody
    • TNFRSF 12A antibody
    • TNFRSF12A antibody
    • TNR12_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 12A antibody
    • TWEAK R antibody
    • Tweak receptor antibody
    • Tweak-receptor antibody
    • TweakR antibody
    see all


  • All lanes : Anti-TWEAKR antibody (ab62496) at 1 µg/ml

    Lane 1 : mock transfected 293 cells
    Lane 2 : Fn14-GPI transfected 293 cells

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 14 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of TWEAKR in human liver tissue with ab62496 antibody at 2.5 µg/ml.

  • Predicted band size : 14 kDa
  • ICC/IF image of ab62496 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62496, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of Fn14 in Human Liver cells using ab62496 at 20 ug/ml.


This product has been referenced in:
  • Affò S  et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut : (2012). IHC-P ; Human . Read more (PubMed: 22637703) »

See 1 Publication for this product

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