Overview

  • Product nameAnti-TWEAKR antibody
    See all TWEAKR primary antibodies
  • Description
    Rabbit polyclonal to TWEAKR
  • SpecificityThis antibody reacts with TWEAKR
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human IgG1 Fc-tagged TWEAKR protein

  • Positive control
    • HepG2 cell lysate, 293 cells and human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 14 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionReceptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • Tissue specificityHighly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • Sequence similaritiesContains 1 TNFR-Cys repeat.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 266 antibody
    • CD266 antibody
    • CD266 antigen antibody
    • FGF inducible 14 antibody
    • FGF-inducible 14 antibody
    • Fibroblast growth factor inducible immediate early response protein 14 antibody
    • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
    • FN 14 antibody
    • FN14 antibody
    • TNFRSF 12A antibody
    • TNFRSF12A antibody
    • TNR12_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 12A antibody
    • TWEAK R antibody
    • Tweak receptor antibody
    • Tweak-receptor antibody
    • TweakR antibody
    see all

Anti-TWEAKR antibody images

  • All lanes : Anti-TWEAKR antibody (ab62496) at 1 µg/ml

    Lane 1 : mock transfected 293 cells
    Lane 2 : Fn14-GPI transfected 293 cells

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of TWEAKR in human liver tissue with ab62496 antibody at 2.5 µg/ml.


  • Predicted band size : 14 kDa
  • ICC/IF image of ab62496 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62496, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of Fn14 in Human Liver cells using ab62496 at 20 ug/ml.

References for Anti-TWEAKR antibody (ab62496)

This product has been referenced in:
  • Affò S  et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut : (2012). IHC-P ; Human . Read more (PubMed: 22637703) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"